Ghost Trap DS - 應用資訊

Mobile phase cleaner for HPLC

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即使在有機溶劑中,也能持續捕集雜質

Ghost Trap DS 的一個主要特色,是即使在有機溶劑中,也能去除雜質。在逆相梯度分析中,當 Ghost Trap DS 安裝在梯度混合器和自動進樣器之間,可以捕集來自管路和梯度混合器的雜質,以及來自移動相的雜質。

以下的範例顯示,Ghost Trap DS 安裝在梯度混合器的下游 (如右圖所示) 時,可以有效捕集移動相中的雜質。

 
Column ODS column
Mobile phase A: 25 mmol/L Phosphate (Potassium) buffer solution pH 4.0 /Acetonitrile =9/1
B: Water/Acetonitrile = 1/9
Flow rate 0.65 mL/min
Oven 45 ºC
Detection UV 210 nm
      Ghost Trap DS 去除幽靈峰範例 (數據由 Daiichi Sankyo Co., Ltd. 提供)

使用 Nexera UHPLC 進行雜質定量分析的應用

對被幽靈峰覆蓋的小峰進行定量可能很困難。Ghost Trap DS/DS-HP 對雜質的定量分析非常有效,可有效消除這一類的幽靈峰,即使目標成分峰非常小,也能確保定量高度準確。

Analytical conditions

Mobile phase A 5 mmol/L phosphoric acid (sodium) buffer solution (pH 2.6)
Mobile phase B 5 mmol/L phosphoric acid (sodium) buffer solution (pH 2.6) / Acetonitrile = 1/3
Gradient 0.1 % (0 min) → 20 % (10 min) → 60 % (15 min) → 95 % (20 min)
Flow rate 0.5 mL/min
Detection UV245 nm
Injection volume 2 μL
Column Shim-pack XR-ODSⅢ 75 mmL. x 2.0 mmI.D., 1.6 μm
 

未使用 Ghost Trap DS/DS-HP 時,雜質峰因為幽靈峰
的影響而很難的定量。TGhost Trap DS/DS-HP 可有效
消除幽靈峰以對目標成分進行定量。