Shim-pack GISS Series - 特色
HPLC Column Solutions
Column Chemistries
| Shim-pack GISS C18 | |
|---|---|
| Bonded Phase | Octadecyl Groups |
| Analysis Mode | Reversed Phase |
| Features | High-speed analysis with ultra-high inertness and high durability |
| Particle Size (μm) | 1.9, 3, 5 |
| Pore Size (nm) | 20 |
| Surface Area (m2/g) | 200 |
| Carbon Loading (%) | 9 |
| End Cap | ○ |
| pH Range | 1-10 |
| USP Code | L1 |
高惰性填充材料可減少如高血壓藥物等鹼性化合物的吸附，並提供良好的峰形。
200Å 的孔徑能夠很好地分離複雜和大分子量的樣品，例如已消化的胜肽。
具有超高惰性和高耐用性的高速分析
分析實例
β-blocker
■Peaks (100 μg/L each)
| Q1 > Q3 | |
| 1. Acebutolol | 337.10 > 116.05 (+) |
| 2. Atenolol | 267.25 > 145.00 (+) |
| 3. Labetalol | 329.00 > 161.95 (+) |
| 4. Nadolol | 310.05 > 254.00 (+) |
| 5. Pindolol | 249.80 > 116.00 (+) |
■Conditions
| Column | Shim-pack GISS C18 (50 mmL x 2.1 mmI.D., 1.9 μm) |
|---|---|
| Eluent | A) 10 mM Ammonium Formate in H2O B) 10 mM Ammonium Formate in CH3OH A/B = 70/30 ‒ 0.3min - 40/60 ‒ 0.5min - 0/100 ‒ 0.1min ‒ 0/100 ‒ 0.01min -70/30 ‒ 0.5min - 70/30, v/v |
| Flow Rate | 0.6 mL/min |
| Col. Temp. | 40 °C |
| Detection | LC/MS/MS（ESI, Positive, Negative MRM） |
| metal-free | Available |